Review



cd31  (R&D Systems)


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    Structured Review

    R&D Systems cd31
    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
    Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd31/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13091142-299-20-21
    Average 98 stars, based on 1248 article reviews
    cd31 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration"

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.04.004

    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
    Figure Legend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Techniques Used: Immunofluorescence, Expressing, Marker

    Related Articles

    Incubation:

    Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema
    Article Snippet: .. For dtTomato visualization, PCLS slides were incubated overnight at 4°C with anti-CD31 (1:200, #AF3628-SP, R&D Systems) and anti-alpha smooth muscle actin (ACTA2; 1:200, #A5228, Sigma-Aldrich) Co-detection of mRNA COX4I2 and protein markers was detected using RNAScope Multiplex FLv2 kit (Bio□Techne). .. Human COX4I2 mRNA (#570351-C3) was detected using the Opal Polaris 7-color manual IHC kit (Akoya Biosciences).

    Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets.
    Article Snippet: .. Slides were then incubated overnight at 4 °C with anti-Insulin (ABclonal, A19066; diluted 1:400), anti-CD31 (RD systems, AF3628-SP; diluted 1:200) or antiCD34 (ABclonal, A19015; diluted 1:200) primary antibody in PBS supplemented with 1% BSA and 0.05% Tween-20 with IgG staining as negative control. .. Slides were washed three times with PBS and then incubated with secondary antibodies (ABclonal, AS014 or AS029) at a 1:500 dilution for 1 h at room temperature.

    RNAscope:

    Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema
    Article Snippet: .. For dtTomato visualization, PCLS slides were incubated overnight at 4°C with anti-CD31 (1:200, #AF3628-SP, R&D Systems) and anti-alpha smooth muscle actin (ACTA2; 1:200, #A5228, Sigma-Aldrich) Co-detection of mRNA COX4I2 and protein markers was detected using RNAScope Multiplex FLv2 kit (Bio□Techne). .. Human COX4I2 mRNA (#570351-C3) was detected using the Opal Polaris 7-color manual IHC kit (Akoya Biosciences).

    Multiplex Assay:

    Article Title: Mitochondrial COX4I2 drives pericyte-dependent inflammation and emphysema
    Article Snippet: .. For dtTomato visualization, PCLS slides were incubated overnight at 4°C with anti-CD31 (1:200, #AF3628-SP, R&D Systems) and anti-alpha smooth muscle actin (ACTA2; 1:200, #A5228, Sigma-Aldrich) Co-detection of mRNA COX4I2 and protein markers was detected using RNAScope Multiplex FLv2 kit (Bio□Techne). .. Human COX4I2 mRNA (#570351-C3) was detected using the Opal Polaris 7-color manual IHC kit (Akoya Biosciences).

    other:

    Article Title: A mouse-adapted Yezo virus model for antiviral testing in immunocompetent mice
    Article Snippet: The commercial primary antibodies used in this study were as follows: anti-albumin (AF3329), anti-CD31 (AF3628), and anti-desmin (AF3844) (R&D Systems, USA); anti-F4/80 (ab320060) (Abcam, USA); anti-NLRP3 (15101S), anti-beta-Actin (4970S), and anti-GAPDH (2118S) (Cell Signaling Technology, USA).

    Article Title: Identification of ephrin-A1–EphA2 signalling as a potential target for fracture prevention
    Article Snippet: The following primary antibodies were used: anti-CD31 (AF3628, R&D systems, 1:100 dilution), anti-RUNX2 (ab192256, Abcam, 1:100), and anti-CD56 (AF2408, R&D systems, 1:100 dilution).

    Article Title: A mouse-adapted Yezo virus model for antiviral testing in immunocompetent mice.
    Article Snippet: The commercial primary antibodies used in this study were as follows: anti-albumin (AF3329), anti-CD31 (AF3628), and anti-desmin (AF3844) (R&D Systems, USA); anti-F4/80 (ab320060) (Abcam, USA); anti-NLRP3 (15101S), anti-beta-Actin (4970S), and anti-GAPDH (2118S) (Cell Signaling Technology, USA).

    Staining:

    Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets.
    Article Snippet: .. Slides were then incubated overnight at 4 °C with anti-Insulin (ABclonal, A19066; diluted 1:400), anti-CD31 (RD systems, AF3628-SP; diluted 1:200) or antiCD34 (ABclonal, A19015; diluted 1:200) primary antibody in PBS supplemented with 1% BSA and 0.05% Tween-20 with IgG staining as negative control. .. Slides were washed three times with PBS and then incubated with secondary antibodies (ABclonal, AS014 or AS029) at a 1:500 dilution for 1 h at room temperature.

    Negative Control:

    Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets.
    Article Snippet: .. Slides were then incubated overnight at 4 °C with anti-Insulin (ABclonal, A19066; diluted 1:400), anti-CD31 (RD systems, AF3628-SP; diluted 1:200) or antiCD34 (ABclonal, A19015; diluted 1:200) primary antibody in PBS supplemented with 1% BSA and 0.05% Tween-20 with IgG staining as negative control. .. Slides were washed three times with PBS and then incubated with secondary antibodies (ABclonal, AS014 or AS029) at a 1:500 dilution for 1 h at room temperature.



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    Image Search Results


    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Journal: Bioactive Materials

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    doi: 10.1016/j.bioactmat.2026.04.004

    Figure Lengend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Article Snippet: After antigen retrieval and blocking, sections were incubated overnight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789 ).

    Techniques: Immunofluorescence, Expressing, Marker